UCR

Neuroscience Graduate Program



Thomas H. Morton


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Office: (951) 827-4735
Fax: (951) 827-4713

Office Hours: , 2pm - 3pm
Email: thomas.morton@ucr.edu

Thomas H. Morton


Biography

Ongoing efforts in this research group are evenly divided between gas-phase ion chemistry (with a view toward being able to predict mass spectra from first principles) and neuroscience-related projects:

  1. Covalent modification of receptor sites involved in vertebrate olfaction. We have demonstrated, by use of a behavioral assay, that it is possible to block selectively the olfactory detection of aldehydes and ketones. This specific odor blindness ("selective hyposmia") is produced by sequential application of acetoacetic ester followed by cyanoborohydride directly to the receptor epithelia of experimental animals and is congruent to chemistry that we have examined for Schiff base-forming proteins in vivo. Using radiolabeled reagents we have isolated a membrane-bound protein that is unique to the olfactory epithelium, which we believe is one receptor type involved in the sense of smell.
  2. An alternative approach for isolation of cell-surface receptors involves biotin-avidin labelling. We seek to attach biotin irreversibly to an olfactory receptor, so that membrane patches containing functional transducing units can be purified. Since cells contain a number of biotinylated proteins to begin with, an avidin affinity column cannot be expected to provide a sufficient degree of purification by itself. We have therefore synthesized a series of silicon-containing extender arms that can be hydrolyzed by catalytic action of fluoride ion. This provides an additional stage of purification: sequestration of biotinylated fractions by avidin as the first stage, followed by selective release of units that are connected to biotin via the fluoridolyzable linker as the second. We call this new technique "two-step affinity chromatography."

Publications

  • Serafin, S.V., Maranan, R., Zhang, K., and Morton, T.H. (2005) "Mass Spectrometric Differentiation of Linear Peptides Composed of L-Amino Acids from Isomeres Containing One D-Amino Acid Residue". Analytical Chemical 77: 5480-5487.
  • Serafin, S.V., Zhang, K., Aurelio, L., Hughes, A.B., and Morton, T.H. (2004) "Decomposition of Protonated Threonine, its Stereoisomeres, and its Homologues in the Gas Phase: Evidence for Internal Backside Displacement." Organic Letters 6: 1561-1564.
  • Morton, T.H. (2004) "Mass Spectrometric Discrimination of Acyclic Stereoisomeres via Competing Unimolecular Decompositions". In The Encyclopedia of Mass Spectrometry, Volume 4 (N.M.M. Nibbering, ed.), Elsevier, London, pp. 433-443.
  • Morton, T.H. (2000) Archiving Odors. In The Philosophy of Chemistry (N. Bhushan and S. Rosenfeld, eds), Oxford University Press, New York, pp. 251-272.
  • Bennett, J.S., Bell, D.W., Buccholz, B.A., Kwok, E.S.C., Vogel, J.S. and Morton, T.H. (1998) Accelerator mass spectrometry for assaying irreversible covalent modification of an enzyme by acetoacetic ester. Int. J. Mass Spectrometry 179/180: 185-193.
  • Taphanel, M.H., Morizur, J.P., Leblanc, D., Borchardt, D., and Morton, T.H. (1997). Stereochemical analysis of monodeuterated isomers by GC/MS/MS. Analytical Chemistry, 69: 4191-4196.
  • Audier, H.E., Bennett, J.S., Kelcher, M.G., and Morton, T.H. (1995). Chiral substitution with isotopic hydrogen: Mass spectrometry and the diastereomer problem. In The Synthesis and Applications of Isotopes and Isotopically Labelled Compounds, Wiley-Interscience, pp. 481-486.

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Cell Biology and Neuroscience
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Ph.D. Program in Neuroscience
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E-mail: neuro@mail.ucr.edu

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